Enviar este artículo por correo electrónico New findings of Heterorhabditis bacteriophora and Steinernema rarum (Nematoda: Heterorhabditidae, Steinernematidae) in Córdoba, Argentina
Resumen
A total of 152 soil samples collected from gardens of private properties at Córdoba city, Argentina, were evaluated for the presence of entomopathogenic nematodes (EPNs). The insect bait method was used to isolate EPNs. The nematodes were characterized using both classic (morphometric characters) and molecular methods (analysis of internal transcribed spacer (ITS) and D2/D3 sequences of 28S genes). The recovery frequency of EPNs was 10.53%. Isolates belonging to the genera Heterorhabditis Poinar, 1976 and Steinernema (Travassos, 1927) were detected. Two isolates were identified as Steinernema rarum and four as Heterorhabditis bacteriophora. Granulometry, relative humidity and pH of the soil were determined and no significant differences were found among sites with presence or absence of EPNs when edaphic characteristics of sites were compared. The occurrence of EPNs in home gardens was determined for the first time, expanding S. rarum and H. bacteriophora currently known geographical distribution and increasing the diversity of populations of these nematodes to use against insect pests of urban areas.
Palabras clave
Texto completo:
PDF (English)Referencias
Adams, B.J., A. Fodor, H.S. Koppenhöfer, E. Stackebrandt, S.P. Stock & M.G. Klein. 2006. Biodiversity and systematic of nematode-bacterium entomopathogens. Biological Control 37(1): 32-49.
Agazadeh, M., D. Mohammadi & N.E. Kary. 2010. Distribution of Entomopathogenic Nematodes of the Families Steinernematidae and Heterorhabditidae in Potato Fields in North-West Iran (Nematoda: Rhabditida). Munis Entomology & Zoology 5(2):758-763.
Alumai, A., P. Grewal, C.W. Hoy & D.A. Willoughby. 2006. Factors affecting the natural occurrence of entomopathogenic nematodes in turfgrass. Biological Control 36(3): 368-374.
Barbosa-Negrisoli, C.R.C., M.S. García, C. Dolinski, A.S. Negrisoli Jr., D. Bernardi & F.J. dos Santos. 2010. Survey of Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae, Steinernematidae) in Rio Grande do Sul State, Brazil. Nematologia Brasileira 34(4): 189-197.
Baur, M.E. & H.K. Kaya. 2001. Persistence of entomopathogenic nematodes. In: M.E. Baur & J. Fuxa (eds.), Environmental persistence of entomopathogens and nematodes, 47 p, Southern Cooperative Series Bulletin 398. Oklahoma Agricultural Experiment Station.
Bedding, R.A. 1990. Logistics and Strategies for Introducing Entomopathogenic Nematode Technology into Developing Countries. In: R. Gaugler & H.K. Kaya (eds.), Entomopathogenic Nematodes in Biological Control, pp. 233-246, CRC Press. Boca Raton, Florida.
Bedding, R.A. & R.J. Akhurst. 1975. A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica 21(1): 109-110.
Bruford, M.W., O. Hanotte, J.F.Y. Brookfield & T. Burke. 1992. Single-locus and multilocus DNA fingerprinting. In: A.R. Hoelzel (ed.), Molecular Genetic Analysis of Populations: A Practical Approach, pp. 225-269, Oxford University Press. New York.
Choo, H.Y., H.K. Kaya & S.P. Stock. 1995. Isolation of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Korea. Japanese Journal of Nematology 25(1): 44-51.
Croll, N.A. & B.E. Matthews. 1977. Survival of nematodes. In: N.A. Croll & B.E. Matthews (eds.), Biology of Nematodes, pp. 152-165, John Wiley and Sons. New York.
Di Rienzo, J.A., F. Casanoves, M.G. Balzarini, L. Gonzalez, M. Tablada & C.W. Robledo. 2011. InfoStat, Versión 2011. Grupo InfoStat. FCA, UNC, Córdoba, Argentina.
Doucet, M.E. & M.M.A. de Doucet. 1997. Nematodes and agriculture in continental Argentina. An overview. Fundamental and Applied Nematology 20(6): 521-539.
Doucet, M.E., M.A. Bertolotti, S.R. Cagnolo & P. Lax. 2008. Nematodos entomofílicos de la provincia de Córdoba, Argentina. Anales de la Academia Nacional de Agronomía y Veterinaria LXII: 263-297.
Ehlers, R.U., K.V. Deseo & E. Stackebrandt. 1991. Identification of Steinernema spp. (Nematoda) and their symbiotic bacteria Xenorhabdus spp. from Italian and German soils. Nematologica 37(1): 360-366.
García del Pino, F. & A. Palomo. 1996. Natural Ocurrence of Entomopathogenic Nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Spanish Soil. Journal of Invertebrate Pathology 68(1): 84-90.
Gaugler, R. & H.K. Kaya. 1990. Entomopathogenic Nematodes in Biological Control. CRC Press, Boca Raton, Florida, 365 pp.
Giayetto, A.L. & L.I. Cichón. 2006. Distribución, gama de huéspedes y especificidad de cinco poblaciones de Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae) del Alto Valle de Río Negro y Neuquén, Argentina. Revista de Investigaciones Agropecuarias 35(2): 163-183.
Glazer, I., N. Liran & Y. Steinberger. 1991. A survey of entomopathogenic nematodes (Rhabditida) in the Negev desert. Phytoparasitica 19(4): 291-300.
Griffin, C.T., J.F. Moore & M.J. Downes. 1991. Occurrence of insect parasitic nematodes (Steinernematidae and Heterorhabditidae) in the Republic of Ireland. Nematologica 37(1): 92-100.
Hatting, J., S.P. Stock & S. Hazir. 2009. Diversity and distribution of the entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in South Africa. Journal of Invertebrate Pathology 102(2): 120-128.
Hazir, S., H.K. Kaya, P. Stock & N. Keskin. 2003. Entomopathogenic Nematodes (Steinernematidae and Heterorhabditidae) for Biological Control of Soil Pests. Turkish Journal of Biology 27(4): 181-202.
Hominick, W.M., 2002. Biogeography. In: R. Gaugler (ed.), Entomopathogenic Nematology, pp. 115-143, Centre for Agriculture and Biosciences International, Wallingford, UK.
Hominick, W.M. & B.R. Briscoe. 1990. Occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in British soils. Parasitology 100(2): 295-302.
Hominick, W.M.A., P. Reid & B.R. Briscoe. 1995. Prevalence and habitat specificity of steinernematid and heterorhabditid nematodes isolated during soil surveys of the UK and the Netherlands. Journal of Helminthology 69(1): 27-32.
Hominick, W.M., A.P. Reid, D.A. Bohan & B.R. Briscoe. 1996. Entomopathogenic nematodes: Biodiversity, Geographical Distribution, and the Convention on Biological Diversity. Biocontrol Science and Technology 6(3): 317-332.
Hominick, W.M., B.R. Briscoe, F. García del Pino, J. Heng, D.J. Hunt, E. Kozodoy, Z. Mracek, K.B. Nguyen, A.P. Reid, S. Spiridonov, P. Stock, D. Sturhan, C. Waturu & M. Yoshida. 1997. Biosystematics of entomopathogenic nematodes: current status, protocols and definitions. Journal of Helminthology 71(4): 271-298.
Hoyos, D. & F. García del Pino. 2004. Presència de nematodes entomopatògens als parcs i jardins de Barcelona i la seva potencialitat en el control de les plagues de la gespa. Barcelona Verda 97: 32-33.
Huelsenbeck, J.P. & F. Ronquist. 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17(8): 754-755.
Kaya, H.K. 1990. Soil Ecology. In: R. Gaugler & H.K. Kaya (eds.), Entomopathogenic Nematodes in Biological Control, pp. 93-115, CRC Press, Boca Raton, Florida.
Kaya, H.K. & S.P. Stock. 1997. Techniques in Insect Nematology. In: L.A. Lacey (ed.), Manual of Techniques in Insect Pathology, pp. 281-324, Academic Press, San Diego, California.
Lacey, L.A. & R. Georgis. 2012. Entomopathogenic Nematodes for Control of Insect Pests Above and Below Ground with Comments on Commercial Production. Journal of Nematology 44(2): 218-225.
López-Núñez, J.C., L. Cano & C.E. Góngora-B. & S.P. Stock. 2007. Diversity and evolutionary relationships of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the Central Andean region of Colombia. Nematology 9(3): 333-341.
López-Núñez, J.C., K. Plichta, C.E. Góngora-Botero & S.P. Stock. 2008. A new entomopathogenic nematode, Steinernema colombiense n. sp. (Nematoda: Steinernematidae), from Colombia. Nematology 10(4): 561-574.
Miduturi, J.S., M. Moens, W.M. Hominick, B.R. Briscoe & A.P. Reid. 1996. Naturally occurring entomopathogenic nematodes in the province of West-Flanders, Belgium. Journal of Helminthology 70(4): 319-327.
Miduturi, J.S., L. Waeyenberge & M. Moens. 1997. Natural distribution of entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) in Belgian soils. Russian Journal of Nematology 5(1): 55-65.
Mráček, Z. & J.M. Webster. 1993. Survey of Steinernematidae and Heterorhabditidae (Rhabditida, Nematoda) in western Canadá. Journal of Nematology 25(4): 710-717.
Nylander, J.A.A. 2004. Mr Model test v2. Program distributed by the author, Evolutionary Biology Centre, Uppsala University.
Prasad, G.S., H.R., Ranganath & P.K. Singh. 2001. Occurrence of the entomopathogenic nematode in parts of south Andamans. Current Science 80(4): 501-502.
Rio, R. & E. Cameron. 2000. Heterorhabditis bacteriophora: seasonal dynamics and distribution in a stand of sugar maple, Acer saccharum. Journal of Invertebrate Pathology 75(1): 36-40.
Román, J. & W. Figueroa. 1995. Morphometric evaluation of 20 Heterorhabditis isolates from Puerto Rico. Journal of Agriculture of the University of Puerto Rico 79(1/2): 51-64.
Seinhorst, J.W. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4(1): 67-69.
Shamseldean, M.M. & M.M. Abd-Elgawad. 1994. Natural occurrence of insect pathogenic nematodes (Rhabditida: Heterorhabditidae) in Egyptian soils. Afro-Asian Journal of Nematology 4(2): 151-154.
Shapiro-Ilan, D.I., W.A. Gardner, J.R. Fuxa, B.W. Wood, K.B. Nguyen, B.J. Adams, R.A. Humber, M.J. Hall. 2003. Survey of entomopathogenic nematodes and fungi endemic to pecan orchards of the Southeastern United States and their virulence to the pecan weevil (Coleoptera: Curculionidae). Environmental Entomology 32(1):187-195.
Simard, L., G. Bélair, S.P. Stock, H. Mauléon & J. Dionne. 2007. Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae) on golf courses in eastern Canada. Nematology 9(3):325-332.
Spiridonov, S.E. & E.A. Guzeeva. 2009. Phylogeny of nematodes of the superfamily Thelastomatoidea (Oxyurida) inferred from LSU rDNA sequence. Russian Journal of Nematology 17(2):127-134.
Stock, S.P. 1995. Natural populations of entomopathogenic nematodes in the Pampean region of Argentina. Nematropica 25(2):143-148.
Stock, S.P. & J.C. Gress. 2006. Diversity and phylogenetic relationships of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the Sky Islands of southern Arizona. Journal of Invertebrate Pathology 92(2):66-72.
Stock, S.P. & H.K. Kaya. 1996. A multivariate analysis of morphometric characters of Heterorhabditis species and the role of morphometrics in the taxonomy of the species of the genus. Journal of Parasitology 82(5):806-813.
Stock, S.P., B. Pryor & H.K. Kaya. 1999. Distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in natural habitats in California, USA. Biodiversity and Conservation 8 (4):535-549.
Stock, S.P., J.F. Campbell & S.A. Nadler. 2001. Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. Journal of Parasitology 87(4):877-889.
Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin & D.G. Higgins. 1997. The CLUSTAL-X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25(24):4876-4882.
Uribe-Lorío, L., M. Mora & S.P. Stock. 2005. First record of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in Costa Rica. Journal of Invertebrate Pathology 88(3):226-231.
Vänninen, I., G.B. Husberg & H.M. hokkanen. 1989. Occurrence of entomopathogenic fungi and entomopathogenic nematodes in cultivated soils in Finland. Acta Entomologica Fennica 53:65-71.
Vrain, T.C., D.A. Wakarchuk, A.C. Lévesque & R.I. Hamilton. 1992. Intraspecific rDNA Restriction Fragment Length Polymorphisms in the Xiphinema americanum Group. Fundamental and appied Nematology 15(6):563-573.
Yoshida, M., A.P. Reid, B.R. Briscoe & W.M. Hominick. 1998. Survey of entomopathogenic nematodes (Rhabditidae: Steinernematidae and Heterorhabditidae) in Japan. Fundamental and applied Nematology 21(2):185-198.
Zepeda-Jazo, I., J. Molina-Ochoa, R. Lezama-Gutierrez, S.R. Skoda & J. E. Foster. 2014. Survey of entomopathogenic nematodes from the families Steinernematidae and Heterorhabditidae (Nematoda: Rhabditida) in Colima, México. International Journal of Tropical Insect Science 34(1):53-57.
Enlaces refback
- No hay ningún enlace refback.
This work is licensed under a Creative Commons Attribution 3.0 License.